Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
hnRNP U (E2O1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP U protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val479 of human hnRNP U protein.
Background
hnRNP U, also known as scaffold attachment factor A (SAF-A) or SP120, was first described as a nuclear matrix protein that contributes to RNA metabolism (1-3). hnRNP U has been shown to bind pre-mRNAs and contributes to splicing by controlling U2 snRNP maturation (4). Furthermore, hnRNP U has been shown to bind chromatin to help regulate its structure during interphase (5). During prophase, hnRNP U becomes phosphorylated by Aurora-B, which evicts hnRNP U and the chromatin-associated RNAs bound to it, a process critical to proper chromosome alignment (6,7). hnRNP U plays a role in the chromatin localization of Xist RNA, and loss of hnRNP U in ES cells fail to achieve an inactivated X chromosome (8). hnRNP U has also been shown to play a key role in immunity, functioning as a viral dsRNA sensor. Upon viral recognition, hnRNP U oligomerizes and activates numerous antiviral genes, including IFNB1 (9). Loss of function variants of hnRNP U have been shown to cause early onset epilepsy and intellectual disorders (10,11).
Alternate Names
C1orf199; EIEE54; GRIP120; Heterogeneous nuclear ribonucleoprotein U; heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A); hnRNP U; hnRNP U protein; HNRNPU; HNRNPU antisense RNA 1; HNRNPU-AS1; HNRPU; Nuclear p120 ribonucleoprotein; p120; p120 nuclear protein; pp120; SAF-A; SAFA; Scaffold attachment factor A; Scaffold-attachment factor A; U21.1
