Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Frozen): 1:1600 - 1:3200
Immunofluorescence (Immunocytochemistry): 1:3200 - 1:6400
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
LAMP2 (E6A6S) Rabbit Monoclonal Antibody recognizes endogenous levels of total LAMP2 protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse LAMP2 protein.
Background
Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6).
Alternate Names
CD107 antigen-like family member B; CD107b; Lam; Lamp; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-B; lysosomal membrane glycoprotein 2; Lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2; lysosome-associated membrane protein-2; Mac; Mac3; OTTMUSP00000018739; OTTMUSP00000018801
