Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
SARS-CoV-2 ORF3a Antibody recognizes endogenous levels of total SARS-CoV-2 ORF3a protein.
Species Reactivity: Virus
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile232 of SARS-CoV-2 ORF3a protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
SARS-CoV-2 ORF3a is the largest of the SARS-CoV-2 accessory proteins containing three amino-terminal transmembrane domains localized to the plasma membrane and perinuclear regions including the golgi apparatus (1). It contains several functional domains including a TRAF3 binding motif, a caveolin-binding motif, a tyrosine-sorting motif, and a diacidic motif (2). SARS-CoV ORF3a was shown to regulate apoptotic and necrotic cell death and inhibit late stages of autophagy by inhibiting fusion of the autophagosome and lysosome (3-5). SARS-CoV-2 ORF3a was also found to inhibit late stages of autophagy by inhibiting fusion of the autophagosome and lysosome through association with components of the homotypic fusion and protein sorting HOPS complex (6-8). It is thought that SARS-CoV-2 can form ion channels that regulates viral release (9). In addition, it can regulate several aspects of innate immune responses, including TRAF3 signaling, activation of NF-κB, and production of inflammatory cytokines (10,11). α
Alternate Names
Accessory protein 3a; AP3A_SARS2; ORF3a protein; Protein 3a; Protein U274; Protein X1; SARS-CoV-2 ORF3a protein
