Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
NRROS (E7Q2J) Rabbit Monoclonal Antibody recognizes endogenous levels of total NRROS protein. A band of unknown origin is detected at 40 kDa in some human cell lines.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Arg136 of human NRROS protein.
Background
Reactive oxygen species (ROS) produced by phagocytes are critical for defense against microbial and fungal challenges. However, excess ROS can lead to tissue damage from inflammation and thus must be carefully regulated (1,2). Negative regulator of ROS (NRROS, LRRC33) limits ROS production in phagocytes during inflammatory responses (1-3). NRROS expression in phagocytes can be repressed by inflammatory signals (2,3). NRROS-deficient phagocytes produce increased ROS upon inflammatory challenge, and mouse phagocytes lacking NRROS show enhanced bactericidal activity (2,3). NRROS has also been shown as a key regulator of transforming growth factor-β1 (TGF-β1) required for microglia function in the nervous system (3,4). Microglia and other macrophages within the central nervous system (CNS) have roles in neural development, inflammation, and homeostasis (3,5). NRROS is required for normal gene expression driving microglial development and function (6-8). Mutation and dysregulation of NRROS in mouse models and humans leads to severe brain disease owing to oxidative tissue damage in the CNS (6-8). NRROS is also involved in Toll-like receptor (TLR) signaling. The ability of NRROS to inhibit NF-κB activation downstream of TLR activation and subsequent decreased cytokine production is likely related indirectly to its role in the TGF-β1 pathway (9).
Alternate Names
ELLP3030; GARPL1; leucine rich repeat containing 33; leucine-rich repeat-containing protein 33; LRC33; LRRC33; MGC50789; Negative regulator of reactive oxygen species; NRROS; SENEBAC; Transforming growth factor beta activator LRRC33; UNQ3030
