Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:200 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
NSUN5 (E5N7R) Rabbit Monoclonal Antibody recognizes endogenous levels of total NSUN5 protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His392 of human NSUN5 protein.
Background
NSUN5 was originally discovered as a yeast 5-methylcytosine methyltransferase that methylates C2278 of the 25S ribosomal RNA (rRNA) (1). It also methylates C3782 in human 28S rRNA, and contributes to protein synthesis and cell growth (2). NSUN5 is overexpressed in many cancer types, where it contributes to increased cellular proliferation (3,4). NSUN5 also plays an important role in cerebral cortex development, and NSUN5 mutants have neurological dysfunction (5-7).
Alternate Names
28S rRNA (cytosine-C(5))-methyltransferase; FLJ10267; MGC986; NOL1; NOL1-related protein; NOL1/NOP2/Sun domain family member 5; NOL1/NOP2/Sun domain family, member 5; NOL1R; NOP2/Sun domain family, member 5; NOP2/Sun domain family, member 5A; NOP2/Sun RNA methyltransferase 5; NOP2/Sun RNA methyltransferase family member 5; NSUN5; NSUN5A; p120; p120(NOL1); Probable 28S rRNA (cytosine-C(5))-methyltransferase; Putative methyltransferase NSUN5; WBSCR20; WBSCR20A; Williams Beuren syndrome chromosome region 20A; Williams-Beuren syndrome chromosomal region 20A protein; Williams-Beuren syndrome critical region protein 20 copy A
