Description
Product Usage Information
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody use in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
The purified antibody is not directed against any known antigen. It functions as an isotype control for rabbit antibodies.
Source / Purification
The antibody was isolated from naive rabbit sera and purified by affinity chromotography. The antibody was conjugated to Alexa Fluor 647 under optimal conditions with a F/P ratio of 2-6.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to F receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
