Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Gab3 (F3W6T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Gab3 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu481 of human Gab3 protein.
Background
Grb2-associated-binding protein 3 (Gab3) is a member of the Grb-associated binder (Gab) family of adaptor proteins that support the assembly of activated signaling complexes through docking and scaffolding functions (1). The Gab family proteins, Gab1-3, contain a conserved N-terminal pleckstrin homology (PH) domain and multiple phosphorylation sites specific for tyrosine kinases (2). The PH-domain interacts with lipids like phosphatidylinositol-3,4,5-triphosphate (PIP3) to mediate membrane recruitment, and the phosphorylated tyrosines act as recruitment sites for SH2 domain-containing proteins (3,4). Gab3 is expressed in hematopoietic lineage cells, including natural killer (NK) cells, mast cells, and memory CD8 T cells (5). Gab3 plays an important role in peripheral NK cell expansion and is essential for IL-2/IL-15 receptor-mediated MAPK signaling in NK cells (6). Gab3 expression is associated with shorter progression-free survival in ovarian cancer and may be a promising biomarker to predict immunotherapeutic responses in lung adenocarcinoma (LUAD) and melanoma (7-9).
Alternate Names
DOS/Gab family member 3; GAB3; Gab3 scaffolding protein; GRB2 associated binding protein 3; GRB2-associated binder 3; GRB2-associated binding protein 3; GRB2-associated-binding protein 3; Growth factor receptor bound protein 2-associated protein 3
