Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Transferrin (E7F4T) Rabbit Monoclonal Antibody recognizes endogenous levels of total transferrin protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg663 of human transferrin protein.
Background
Transferrin is the major ferric iron transport protein, which binds ferric (Fe3+) ions in association with the binding of an anion, usually bicarbonate (1). The iron binding affinity of transferrin is pH dependent. In a neutral pH environment, transferrin (apotransferrin) binds iron with high affinity to form iron-bound transferrin (holotransferrin). In an acidic pH environment, the affinity of iron bound to transferrin decreases, dissociating iron from holotransferrin and releasing it into the environment (2). The importance of holotransferrin is to sequester Fe3+ ions in a relatively nonreactive and inert state to ensure normal free iron homeostasis in the body (3). Transferrin has been shown to contribute to iron-related oxidative damage (4), DNA synthesis (5), cell growth (6), and differentiation (7). Holotransferrin delivers iron to cells by binding to transferrin receptor (TFR1 or TFR2). Neutral pH at the cell surface promotes binding of holotransferrin to TFR1/TFR2. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH results in the release of iron. The receptor-ligand complex is recycled to the cell surface, where apotransferrin dissociates from TFR1/TFR2 (8).
Alternate Names
Beta-1 metal-binding globulin; DKFZp781D0156; epididymis secretory sperm binding protein Li 71p; HEL-S-71p; PRO1557; PRO2086; Serotransferrin; Siderophilin; TF; TFQTL1; Transferrin; TRFE
