Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
SMG6 (F6A1U) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMG6 protein.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala125 of human SMG6 protein.
Background
Nonsense-mediated mRNA decay (NMD) is a process by which prematurely terminated transcripts are degraded before they can be translated (1-3). There are two differential processes for NMD in higher eukaryotes, endonucleolytic and exonucleolytic, which are governed by SMG6 (also known as EST1A) or SMG5 and SMG7, respectively (1,4-8). SMG5, SMG6, and SMG7 are structurally similar, but SMG6 contains a PIN domain with endonucleolytic activity. SMG5 contains a similar domain, but lacks critical residues for endonuclease activity. SMG7 does not contain a PIN domain (7). All three proteins are recruited to UPF1 when SMG1 phosphorylates it, and the NMD process significantly diverges later. Upon binding to UPF1, SMG5 and SMG7 can recruit decaying and deadenylating protein complexes, resulting in subsequent mRNA degradation via the XRN1 exonuclease and the exosome (8,9). SMG6, however, promotes decay through cleaving nonspecific sites within 40 nucleotides of the premature termination codon (PTC) (7,10). Interestingly, SMG6-mediated decay depends on either a functional SMG5 or SMG7 (11). NMD pathways are species-specific, with relying more on SMG6-mediated decay and yeast utilizing the decaying and deadenylating approach to mRNA turnover (12).
Alternate Names
C17orf31; EST1 telomerase component homolog A; EST1-like protein A; EST1A; Est1p-like; Ever shorter telomeres 1A; hSmg5/7a; KIAA0732; SMG-6; Smg-6 homolog; smg-6 homolog, nonsense mediated mRNA decay factor; Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans); SMG6; SMG6 nonsense mediated mRNA decay factor; Telomerase subunit EST1A; Telomerase-binding protein EST1A
