Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
UBF (F2F1O) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBF protein, including both isoforms UBF1 and UBF2.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBF protein.
Background
RNA polymerase I (RNAPI) is a large multi-protein complex that functions as a DNA-dependent, RNA polymerase, which is primarily responsible for the transcription of ribosomal RNA (rRNA) genes. The largest subunit, Rpa194, or POLR1A, along with selectivity factor 1 (SL1) and the transactivator protein upstream binding factor (UBF), makes up the core transcriptional machinery of the RNAPI complex (1-3). UBF is a nucleolar protein that functions as a dual transcription factor and epigenetic remodeler to promote rRNA transcription (4). ChIP-seq studies show that UBF binds ribosomal DNA (rDNA) at the promoter sequence and throughout the transcribed regions (4,5). At the promoter region, UBF associates with SL1 to drive formation of the preinitiation complex (PIC) (6,7). UBF also promotes rRNA transcription by displacing linker histone H1 and keeping rDNA in an active euchromatin state (8,9). Due to its critical role in ribosome biogenesis, defects in UBF have been linked to several ribosomopathies and neurological disorders (10). Specifically, a gain-of-function E210K mutation in UBF has been linked to multiple cases of pediatric neurodegeneration (11).
Alternate Names
90-kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; CONDBA; NOR-90; Nucleolar transcription factor 1; UBF; UBF-1; UBF1; UBF2; UBTF; upstream binding transcription factor; upstream binding transcription factor, RNA polymerase I; Upstream-binding factor 1
