Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Flow Cytometry (Live): 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 97174 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Live)
Specificity / Sensitivity
NCAM1 (CD56) (123C3) Mouse Monoclonal Antibody detects endogenous levels of total CD56 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing BALB/c mice with a membrane preparation of a small cell lung carcinoma specimen.
Background
NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension, and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms' tumor, neuroblastoma, and rhabdomyosarcoma (2). CD56 is commonly used along with CD3 and CD16 to identify human natural killer (NK) cells (mouse NK cells do not express CD56) (3). Human NK cells are CD3-CD56+. The large subset with high CD16 expression are mature cytotoxic NK cells, while those with low CD16 expression are immature precursors and cytokine producers (4,5).
Alternate Names
antigen recognized by monoclonal antibody 5.1H11; CD56; MSK39; N-CAM-1; NCAM; NCAM-1; NCAM1; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM
