Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:50
Immunofluorescence (Immunocytochemistry): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 36890 .
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Grp75 (D13H4) Rabbit Monoclonal Antibody detects endogenous levels of total Grp75 protein.
Species Reactivity: Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Grp75 protein.
Background
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).
Alternate Names
75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; catecholamine-regulated protein 40; CRP40; CSA; epididymis secretory sperm binding protein Li 124m; EVPLS; GRP-75; GRP75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9; heat shock 70kDa protein 9 (mortalin); heat shock protein family A (Hsp70) member 9; HEL-S-124m; HSPA9; HSPA9B; MGC4500; Mortalin; mortalin-2; mortalin, perinuclear; mortalin2; MOT; mot-2; MOT2; mt-HSP70; MTHSP75; p66-mortalin; PBP74; Peptide-binding protein 74; SAAN; SIDBA4; Stress-70 protein, mitochondrial
