Description
Product Usage Information
Flow Cytometry (Fixed/Permeabilized): 1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Available protocols: Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
p16 INK4A (F2T7H) Rabbit Monoclonal Antibody (Alexa Fluor
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length mouse p16 INK4A protein. This antibody recognizes residues surrounding Arg156 of mouse p16 INK4A protein.
Background
Members of the INK4 family of cyclin-dependent kinase inhibitors include p16 INK4A, p15 INK4B, p18 INK4C, and p19 INK4D. The INK4 family members inhibit cyclin-dependent kinases 4 and 6 (CDK4 and CDK6), causing cell cycle arrest in G1 phase. The INK4A-ARF-INK4B locus on chromosome 9p21, frequently lost in human cancer, encodes the INK4 family members p16 INK5A and p15 INK4B, as well as the unrelated protein, ARF (1). p16 INK4A expression, typically repressed in the absence of stress, is thought to drive cells into senescence, and p16 INK4A expression is a commonly used marker of senescent cells (2,3). p16 INK4A protein expression is often altered in human cancer, and high expression is currently used as a predictive biomarker in cervical cancer (3-5).
Alternate Names
alternative reading frame; Arf; ARF-; ARF-INK4a; CD2A1; CDK4I; Cdkn2a; CDN2A; cyclin dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A, isoform 3; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a-ARF; Ink4a/Arf; mitochondrial smARF; MTS; MTS1; OTTMUSP00000008143; p16; p16-INK4; p16-INK4a; p16(INK4a); p16I; P16ink4a; p19; p19; p19ARF; Pct; Pctr1
