Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
CDC73 (D410) Antibody detects endogenous levels of total CDC73 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp410 of human CDC73. Antibodies are purified by peptide affinity chromatography.
Background
CDC73 (HPRT2) is a putative tumor suppressor protein thought to bind RNA polymerase II to help inhibit cell cycle progression (1,2). Commonly referred to as parafibromin, CDC73 is expressed in endocrine tissues, kidney, heart, and skeletal muscle and is localized to both nuclear and cytoplasmic compartments (3). CDC73 acts as a Wnt signaling regulator as it binds the carboxy-terminal region of β-catenin (4). Mutations in the corresponding gene cause an endocrine disorder known as hyperparathyroidism 2, which is characterized by hypercalcemia, bone resorption, and the development of jaw tumors (5).
Alternate Names
C1orf28; CDC73; cell division cycle 73; cell division cycle 73 Paf1/RNA polymerase II complex component-like protein; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog; cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae); Cell division cycle protein 73 homolog; Familial isolated hyperparathyroidism; FIHP; FLJ23316; HPTJT; HRPT1; HRPT2; Hyperparathyroidism 2 protein; HYX; Paf1/RNA polymerase II complex component; Parafibromin
