Description
Product Usage Information
Immunohistochemistry (Paraffin): 1:125 - 1:500
Immunofluorescence (Frozen): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57834 .
Protocol
Available protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen)
Specificity / Sensitivity
MUC5AC (E9V1O) Rabbit Monoclonal Antibody recognizes endogenous levels of total MUC5AC protein.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys317 of murine MUC5AC protein.
Background
Mucins are a family of macromolecules that line and protect the respiratory epithelium from microbes and pollutants in the local environment. Of the family members that are known to date, some are produced in a cell type and tissue-specific manner, suggesting distinct biological roles for members. Some members polymerize after secretion to form gel-like substances that coat the epithelial layer. MUC5AC and MUC5B are members of the family that polymerize in this manner. Others do not polymerize, and others yet, have a transmembrane domain and remain physically attached to the epithelia (1). While it is known that mucins are protective to the respiratory epithelium, it has been reported that changes in expression of mucins are associated with several forms of lung disease such as cystic fibrosis, COPD, asthma, pulmonary fibrosis, and others (1-4). Multiple epithelial malignancies have been described to show changes in expression, localization, and glycosylation of MUC5AC. This wide association with multiple malignancy types has led to the emergence of MUC5AC as both a prognostic and therapeutic target for cancer (5).
Alternate Names
2210005L13Rik; gastric mucin; MGM; Muc5ac; Mucin 5, subtypes A and C, tracheobronchial/gastric; mucin-5AC; Secreted gel-forming mucin
