Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:50
Immunofluorescence (Immunocytochemistry): 1:50
Flow Cytometry (Fixed/Permeabilized): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
Acetyl-CoA Carboxylase Antibody detects endogenous levels of all isoforms of acetyl CoA carboxylase protein.
Species Reactivity: Human, Mouse, Rat, Monkey, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl CoA carboxylase alpha1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).
Alternate Names
ACAC; ACACA; ACACAD; ACACB; ACC; ACC-alpha; ACC-beta; ACC1; ACC2; ACCA; ACCB; Acetyl-CoA carboxylase 1; Acetyl-CoA carboxylase 2; acetyl-CoA carboxylase alpha; acetyl-CoA carboxylase beta; acetyl-CoA carboxylase-alpha; acetyl-Coenzyme A carboxylase alpha; acetyl-Coenzyme A carboxylase beta; Biotin carboxylase; HACC275
