Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Kindlin-1 (D1K4C) Rabbit Monoclonal Antibody recognizes endogenous levels of total Kindlin-1 protein. This antibody does not cross-react with Kindlin-2 or Kindlin-3.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human Kindlin-1 protein.
Background
The kindlin family of focal adhesion proteins is involved in multiple biological processes, including integrin signaling, adhesion, migration, angiogenesis, differentiation, and mitotic spindle formation (1,2). Kindlin family members 1, 2, and 3 (FERM1, FERM2, and URP2) are differentially expressed in tissues. Kindlin-1 is primarily expressed in epithelial cells, kindlin-2 is ubiquitously expressed, and kindlin-3 expression is restricted to the hematopoietic system (3).
Alternate Names
C20orf42; DTGCU2; FERM1; Fermitin family homolog 1; fermitin family homolog 1 (Drosophila); fermitin family member 1; FERMT1; FLJ20116; FLJ23423; KIND1; Kindlerin; kindlin 1; Kindlin syndrome protein; Kindlin-1; Unc-112-related protein 1; UNC112 related protein 1; UNC112A; URP1
