Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
MCT/SLC16A1 (E7F6Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total MCT1/SLC16A1 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human MCT1/SLC16A1 protein.
Background
MCT1, also known as MOT1, is a multi-pass transmembrane monocarboxylate transporter (MCT) protein, encoded by the gene (1,2). MCT1 is reported to be expressed in multiple tissues and cell types, including erythrocytes, immune cells, and both cardiac and skeletal myocytes. The primary role of MCT1 is to facilitate proton-coupled transport of monocarboxylates (e.g., lactate, pyruvate, ketone bodies) across the plasma membrane in response to cellular metabolic demands (1). The regulation of monocarboxylate flux by MCT1 has been identified as a possible metabolic biomarker in various diseases, including neurodegenerative diseases, ischemia, and cancer (2-5). Inhibition of MCT1 has thus been proposed as a potential therapeutic treatment of cancer (5,6).
Alternate Names
FLJ36745; HHF7; MCT; MCT 1; MCT1; MCT1D; MGC44475; Monocarboxylate transporter 1; MOT1; SLC16A1; solute carrier family 16 (monocarboxylate transporter), member 1; solute carrier family 16 (monocarboxylic acid transporters), member 1; Solute carrier family 16 member 1; solute carrier family 16, member 1; solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
