Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Immunofluorescence (Immunocytochemistry): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
eEF2k Antibody detects endogenous levels of total eEF2k independent of phosphorylation.
Species Reactivity: Human, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the N-terminus of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.
Alternate Names
alternative protein EEF2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; CaMKIII; eEF-2 kinase; eEF-2K; EEF2K; EF2K; elongation factor 2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; HSU93850; MGC45041
