Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Immunohistochemistry (Paraffin): 1:50 - 1:200
Immunofluorescence (Immunocytochemistry): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ZNF185 (E6W4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZNF185 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly50 of human ZNF185 protein.
Background
Zinc finger protein 185 (ZNF185) is an actin cytoskeleton-associated protein that regulates anchorage dependent growth of prostate cancer cells (1). ZNF185 expression is regulated by p63 and p53 (2,3). ZNF185 localization and expression was found to have prognostic value in epithelial cancer and pancreatic ductal carcinoma (2,4), and its overexpression reduces proliferation and invasion of lung adenocarcinoma cells (5).
Alternate Names
LIM domain protein ZNF185; P1-A; SCELL; sciellin like; Zinc finger protein 185; zinc finger protein 185 (LIM domain); zinc finger protein 185 with LIM domain; ZN185; ZNF185
