Description
Product Usage Information
Western Blotting: 1:1000
Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
PR3 (F5M5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total PR3 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro145 of human PR3 protein.
Background
Proteinase 3 (PR3 or PRTN3), also known as myeloblastin, is a serine protease primarily expressed in neutrophils (1). PR3 can be localized to both azurophilic granules and the plasma membrane, where it serves as a target of antineutrophil cytoplasmic autoantibodies (ANCA) in the autoimmune condition granulomatosis with polyangiitis, formerly known as Wegener's granulomatosis (2). PR3 can degrade elastin, a component of the extracellular matrix, and activate platelets, dendritic cells, and endothelial cells by interacting with the proteinase-activated receptors (PARs), PAR-1 and PAR-2 (3-6). PR3 is also capable of processing inflammatory cytokines, including TNF-α, IL-1β, and IL-18 (7). PR3 has recently been shown to play a role in hematopoietic differentiation by participating in the cleavage of histone H3. Through its ability to modulate Stat3 to promote myeloid differentiation, it may serve as a therapeutic target in acute myeloid leukemia (7,8).
Alternate Names
ACPA; AGP7; azurophil granule protein 7; C-ANCA; C-ANCA antigen; CANCA; Leukocyte proteinase 3; MBN; MBT; Myeloblastin; Neutrophil proteinase 4; NP-4; NP4; P29; PR-3; PR3; proteinase 3; PRTN3; serine proteinase, neutrophil; Wegener autoantigen; Wegener granulomatosis autoantigen
