Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Plexin A1 Antibody detects endogenous levels of total Plexin A1 protein.
Species Reactivity: Human, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Gln1875 of human Plexin A1. Antibodies are purified by peptide affinity chromatography.
Background
Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3).
Alternate Names
DKFZp761P19121; NOV; NOVP; plexin 1; plexin A1; Plexin-A1; PLXA1; PLXN1; PLXNA1; Semaphorin receptor NOV
