Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
FANCM (E5Y9H) Rabbit Monoclonal Antibody recognizes endogenous levels of total FANCM protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FANCM protein.
Background
Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). FANCB is an X-linked member of the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM). In response to DNA damage, the FA nuclear complex induces mono-ubiquitination of FANCD2 and FANCI (2). FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are then recruited to sites of DNA damage along with other DNA repair proteins. FA signaling is important in maintenance of chromosome stability and control of mitosis (3). Studies of FANCB knockout embryonic stem cells suggest a role for FANCB in the formation of Rad51 and FANCD2 foci at chromosomal sites of DNA damage (4).
Alternate Names
ATP-dependent RNA helicase FANCM; FA complementation group M; FAAP250; FANCM; Fanconi anemia complementation group M; Fanconi anemia group M protein; Fanconi anemia-associated polypeptide of 250 kDa; Fanconi anemia, complementation group M; KIAA1596; MGC176453; POF15; Protein FACM; Protein Hef ortholog; SPGF28
