Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Transportin-1 Antibody recognizes endogenous levels of total transportin-1 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala354 of human transportin-1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Transportin-1 (TNPO1), also known as MIP or karyopherin-β2, is a nuclear import protein that binds to nuclear localization signals (NLSs) on various proteins to help shuttle them from the cytoplasm to the nucleus (1-3). While initially discovered to shuttle hnRNP proteins, transportin-1 has also been shown to import other important regulatory proteins, such as c-Fos and ADAR1 (1,4,5). Transportin-1 plays a role in HIV replication, as it imports and helps uncap the capsid protein (6). Transportin-1 has been implicated in familial ALS, due to its impaired binding affinity to mutations in the FUS-NLS region. Reduction in transportin-1 binding correlated with the time of ALS disease progression in these mutants (7). Transportin-1 can also accumulate in FUS inclusions in wild-type FUS ALS (8). Additionally, transportin-1 plays a role in circadian rhythms by importing PER1 to the nucleus (9).
Alternate Names
importin 2; importin beta 2; Importin beta-2; IPO2; karyopherin (importin) beta 2; Karyopherin beta-2; KPNB2; M9 region interaction protein; MIP; MIP1; TNPO1; transportin 1; Transportin-1; TRN
