Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:1000 - 1:4000
Immunofluorescence (Immunocytochemistry): 1:200 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
ILF3 (E5H6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total ILF3 protein. Species reactivity for IHC-P is human only.
Species Reactivity: Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val353 of human ILF3 protein.
Background
ILF2 and ILF3, also known as NF45 and NF90 respectively, form a double-stranded RNA binding heterodimer that serves many different biological functions. ILF2 and ILF3 were originally discovered as proteins that can bind and regulate the antigen receptor response element of the IL2 promoter to contribute to T cell activation (1,2). ILF2 and ILF3 play important roles in DNA damage response and non-homologous end joining (NHEJ) by interacting with DNA-PK (3,4). The heterodimer can also bind and negatively regulate microRNAs (5,6). Biogenesis of the 60S ribosomal subunit has been shown to be dependent on the double-stranded RNA-binding domains of ILF3 (7). Additionally, ILF2 and ILF3 can mediate rDNA transcription (8). ILF2 and ILF3 also play a role in the life cycle of many different viruses, including dengue and hepatitis C (9-11).
Alternate Names
CBTF; Double-stranded RNA-binding protein 76; double-stranded RNA-binding protein, 76 kD; DRBF; DRBP76; dsRNA binding protein NFAR-2/MPP4; ILF3; interleukin enhancer binding factor 3; interleukin enhancer binding factor 3, 90kD; interleukin enhancer binding factor 3, 90kDa; Interleukin enhancer-binding factor 3; M phase phosphoprotein 4, nuclear factor associated with DS RNA; M-phase phosphoprotein 4; MMP4; MPHOSPH4; MPP4; MPP4110; NF-AT-90; NF110; NF110b; NF90; NF90a; NF90b; NF90c; NF90ctv; NFAR; NFAR-1; NFAR-2; NFAR110; NFAR2; NFAR90; NFAT90; Nuclear factor associated with dsRNA; nuclear factor of activated T-cells 110 kDa; Nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; TCP110; TCP80; Translational control protein 80
