Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunoprecipitation: 1:100
Immunofluorescence (Frozen): 1:50
Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200
Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
p21 Waf1/Cip1 (F2C7C) Rabbit mAb recognizes endogenous levels of total p21 Waf1/Cip1 protein. This antibody detects an approximately 60 kDa protein of unknown identity in some cell lines. Nonspecific staining within the vesicles and mitochondria of immune cells may be observed by immunofluorescence.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length mouse p21 Waf1/Cip1 protein.
Background
The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).
Alternate Names
CAP; CAP20; CDK; CDK-interacting protein 1; CDKI; Cdkn; Cdkn1; Cdkn1a; CDN1A; Cip1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); mda; mda6; Melanoma differentiation-associated protein; p21; p21; p21C; p21Cip1; p21W; p21WAF; SDI1; Waf; Waf1
