Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
p21 Waf1/Cip1 (F7S9M) Rabbit Monoclonal Antibody recognizes endogenous levels of total p21 Waf1/Cip1 protein. This antibody detects approximately 15 kDa and 105 kDa proteins of unknown identity in some cell lines.
Species Reactivity: Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro11 of mouse p21 Waf1/Cip1 protein.
Background
The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).
Alternate Names
CAP; CAP20; CDK; CDK-interacting protein 1; CDKI; Cdkn; Cdkn1; Cdkn1a; CDN1A; Cip1; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (P21); mda; mda6; Melanoma differentiation-associated protein; p21; p21; p21C; p21Cip1; p21W; p21WAF; SDI1; Waf; Waf1
