Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
GRB2 Antibody detects endogenous levels of total GRB2 protein.
Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human GRB2. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Growth factor receptor-binding protein 2 (GRB2) is an adaptor protein that is involved in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins, such as EGFR, IRS-1, Shc, and Gab1 (1). The SH3 domain of GRB2 associates with SOS, which stimulates the Ras GTP-binding activity, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of GRB2 at Tyr209 by Bcr-Abl and EGFR abolishes its association with SOS and negatively regulates downstream signaling (2).
Alternate Names
abundant SRC homology; Adapter protein GRB2; ASH; EGFRBP-GRB2; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; GRB2; Grb3-3; growth factor receptor bound protein 2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; MST084; MSTP084; NCKAP2; Protein Ash; SH2/SH3 adapter GRB2
