Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
LIN28A (P22) Antibody detects endogenous levels of total LIN28A protein.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Pro22 of human LIN28A. Antibodies are purified by protein A and peptide affinity chromatography.
Background
LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).
Alternate Names
CSDD1; FLJ12457; LIN-28; lin-28 homolog A; lin-28 homolog A (C. elegans); Lin-28A; LIN28; LIN28A; LN28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; ZCCHC1; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
