Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Troponin I Antibody detects endogenous levels of total troponin I, both skeletal muscle and cardiac isoforms.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to the sequence of human troponin I. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca concentration. Troponin consists of three subunits: the Ca -binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca sensitivity by reducing the Ca binding affinity of TnC (1-3).
Alternate Names
Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); CMD1FF; CMD2A; CMH7; cTnI; familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; TNNC1; TNNI3; troponin I type 3 (cardiac); Troponin I, cardiac muscle; troponin I3, cardiac type
