Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
MST3b Antibody recognizes endogenous levels of total MST3b protein. This antibody does not cross-react with MST3, MST1, MST2, or MST4 proteins.
Species Reactivity: Human, Mouse, Rat, Monkey, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human MST3b protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Mammalian sterile-20-like (MST) kinases are upstream regulators of mitogen-activated protein kinase (MAPK) signaling pathways that regulate multiple biological processes, including apoptosis, morphogenesis, cell migration, and cytoskeletal rearrangements (1). This group of serine/threonine kinases includes a pair of closely related proteins (MST1, MST2) that are functionally distinct from the more distantly related MST3 and MST4 kinases. All four MST kinases share a conserved amino-terminal kinase domain and carboxy-terminal regulatory and interaction domains (1-3). At least three of these kinases (MST1-3) promote apoptosis and are activated by caspase cleavage, followed by nuclear translocation of the active kinase. MST1/2 kinases play a key role in the Hippo signaling pathway, an evolutionarily conserved program that controls organ size by regulating cell proliferation, apoptosis, and stem cell self-renewal (4). Mammalian Sterile 20-like kinase 3 (MST3, STK24) is ubiquitously expressed as a longer MST3b isoform and a shorter MST3a protein lacking a portion of the amino-terminal region (5). MST3a protein regulates neuronal migration during CNS development, cell motility, and apoptosis (6,7). MST3 phosphorylates and activates the NDR protein kinases that regulate cell cycle progression and cell morphology (8). Autophosphorylation of MST3 at Thr178 is required for kinase activity, and alteration of this residue inhibits MST3 regulation of cell migration (7). The brain-specific MST3b protein is activated by nerve growth factor or inosine and localizes to neurons where it helps regulate axon growth and regeneration (9).
Alternate Names
epididymis secretory protein Li 95; HEL-S-95; Mammalian STE20-like protein kinase 3; Mammalian STE20-like protein kinase 3 C-terminal; Mammalian STE20-like protein kinase 3 N-terminal; MST-3; MST3; MST3/C; MST3/N; MST3B; serine/threonine kinase 24; serine/threonine kinase 24 (STE20 homolog, yeast); Serine/threonine-protein kinase 24; Serine/threonine-protein kinase 24 12 kDa subunit; Serine/threonine-protein kinase 24 36 kDa subunit; STE20; STE20-like kinase 3; STE20-like kinase MST3; sterile 20-like kinase 3; STK24; STK3
