Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Pan-Cadherin (28E12) Rabbit Monoclonal Antibody detects endogenous levels of total cadherin proteins and has a preference for N-, R-, and E-Cadherin. This antibody does not prefer P- or VE- Cadherin.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a conserved region of human N-, R-, and, E-Cadherin.
Background
Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).
Alternate Names
ACOGS; Arc-1; ARVD14; BCDS1; CAD4; CADH1; CADH2; CADH3; CADH4; Cadherin; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin 2; cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); cadherin 3; cadherin 3, type 1, P-cadherin (placental); cadherin 4; cadherin 4, type 1, preproprotein; cadherin 4, type 1, R-cadherin (retinal); Cadherin E; Cadherin-1; Cadherin-2; Cadherin-3; Cadherin-4; calcium-dependent adhesion protein, epithelial; calcium-dependent adhesion protein, neuronal; calcium-dependent adhesion protein, placental; CAM 120/80; CD324; CD325; CDH1; CDH2; CDH3; CDH4; CDHE; CDHN; CDHP; CDw325; cell-CAM 120/80; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; E-cadherin; E-cadherin 1; ECAD; epididymis secretory sperm binding protein; Epithelial cadherin; FLJ22202; FLJ40547; HJMD; LCAM; MGC126700; MGC138355; N Cadherin; N-cadherin; N-cadherin 1; NCAD; Neural cadherin; neural-cadherin; P-cadherin; PCAD; Placental cadherin; R-CAD; R-cadherin; RCAD; Retinal cadherin; UVO; Uvomorulin
