Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
GEF-H1 (55B6) Rabbit Monoclonal Antibody detects endogenous levels of total GEF-H1 protein.
Species Reactivity: Human, Mouse, Rat, Hamster, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GEF-H1.
Background
Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). Phosphorylation of GEF-H1 at Ser886 (Ser885 in mouse), a site located in the 14-3-3 binding motif, has been implicated in recruitment of 14-3-3 and GEF-H1 to microtubules (3), and in the regulation of RhoA activity in response to mitotic kinases during cytokinesis (4). GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability (5,6). GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest (6).
Alternate Names
ARHG2; ARHGEF2; DKFZp547L106; DKFZp547P1516; FLJ45440; GEF; GEF-H1; GEFH1; Guanine nucleotide exchange factor H1; KIAA0651; Lfc; LFP40; Microtubule-regulated Rho-GEF; NEDMHM; P40; Proliferating cell nucleolar antigen p40; Rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2; Rho/Rac guanine nucleotide exchange factor 2; RhoGEF2
