Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody .
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
mNeonGreen Tag (E6M3D) Rabbit Monoclonal Antibody detects exogenously expressed mNeonGreen protein. This antibody does not cross-react with green fluorescent protein (GFP).
Species Reactivity: All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu45 of mNeonGreen protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. mNeonGreen is a 220 amino acid, bright green fluorescent protein derived from . mNeonGreen is a very useful tool as a fusion protein that reports gene expression, traces cell lineage, and defines subcellular protein localization (1).
