Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
CHMP4B Antibody recognizes endogenous levels of total CHMP4B protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn219 of human CHMP4B protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CHMP4B, the human homologue of yeast Snf7, interacts with Alix and is a core component of the ESCRT-III complex (endosomal sorting for transport complex III) (1,2). The ESCRT system is comprised of multiple protein complexes denoted as ESCRT-0, -I, -II, -III, and VPS4 ATPase that together contribute to a number of membrane fission events, including multivesicular body (MVB) and exosome formulation, viral budding, abscission during cytokinesis, and plasma membrane repair (3,4). Interestingly, it was found that the ESCRT-III complex, including CHMP4B is recruited to the plasma membrane during the regulated process of necrosis, necroptosis, and thereby controls membrane integrity sustaining these cells and allowing continued signaling and cytokine/chemokine secretion (5). Elevated expression of CHMP4B has been associated with neuronal apoptosis following intracerebral hemorrhage (6). In addition, high CHMP4B expression was associated with increased proliferation and resistance to doxorubicin in hepatocellular carcinoma (7).
Alternate Names
C20orf178; Charged multivesicular body protein 4b; CHM4B; CHMP4A; CHMP4B; chromatin modifying protein 4B; Chromatin-modifying protein 4b; CTPP3; CTRCT31; dJ553F4.4; hSnf7-2; hVps32-2; SHAX1; SNF7; SNF7 homolog associated with Alix 1; Snf7 homologue associated with Alix 1; SNF7-2; Vacuolar protein sorting-associated protein 32-2; vacuolar protein-sorting-associated protein 32-2; Vps32-2; VPS32B
