Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
Fer (5D2) Mouse Monoclonal Antibody detects endogenous levels of total Fer protein. This antibody does not cross-react with other related proteins.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a truncated recombinant Fer protein.
Background
Fer is a nonreceptor protein-tyrosine kinase of the Fes/Fps family. Like many other cytoplasmic tyrosine kinases, Fer contains a long amino-terminal domain, a central SH2 domain, and a carboxy-terminal kinase domain. Its amino-terminal domain is responsible for protein oligomerization as well as interaction with cytoskeletal proteins. Fer is ubiquitously expressed in a wide variety of cell and tissue types, and is localized to both cytoplasm and nucleus (1). Tyrosine kinase activity of Fer can be stimulated by growth factors and cytokines (2,3). After activation, Fer can further activate various downstream signaling components including Stat3 (3). Fer plays an important role in regulation of cell movement, oncogenesis and inflammation (4).
Alternate Names
Feline encephalitis virus-related kinase FER; FER; fer (fps/fes related) tyrosine kinase; FER tyrosine kinase; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; p94-Fer; phosphoprotein NCP94; PPP1R74; protein phosphatase 1, regulatory subunit 74; Proto-oncogene c-Fer; TYK3; Tyrosine kinase 3; Tyrosine-protein kinase Fer
