Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Ron (1B5) Mouse Monoclonal Antibody detects endogenous levels of the 170 kDa full-length Ron protein. This antibody does not cross-react with other family members.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant Trx-Ron fusion protein containing amino acids 210-320 of human Ron expressed in .
Background
Ron is a member of the Met proto-oncogene family of receptor tyrosine kinases, which also includes Stk, c-Met, and c-Sea. The functional Ron is a heterodimer composed of a 40 kDa α chain and a 150 kDa β chain. Ron is initially synthesized in the cells as a single-chain, pro-Ron precursor that is cleaved into the two active chains. The α chain is completely extracellular, whereas the β chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements (1,2). Ron mediates multiple signaling cascades that involve cell motility, adhesion, proliferation, and apoptosis. The signaling pathways activated downstream of Ron include the ras/mitogen-activated protein kinase (MAPK), phosphatidyl inositol-3 kinase (PI3K)/Akt, and focal adhesion kinase (FAK) pathways. Ron activation can also significantly increase c-Src activity, a signaling intermediate involved in cell cycle progression, motility, angiogenesis, and survival (3,4). The function of Ron has been shown to be important for embryological development as well as implicated in the progression and metastasis of tumors (5).
Alternate Names
c-met-related tyrosine kinase; CD136; CDw136; macrophage stimulating 1 receptor; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); Macrophage-stimulating protein receptor; Macrophage-stimulating protein receptor alpha chain; Macrophage-stimulating protein receptor beta chain; MSP receptor; MST1R; NPCA3; p185-Ron; Protein-tyrosine kinase 8; PTK8; PTK8 protein tyrosine kinase 8; RON; S13 avian erythroblastosis oncogene homolog; S13 erythroblastosis oncogene homolog; SEA; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4
