Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Frozen): 1:200 - 1:400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen)
Specificity / Sensitivity
Kir4.1 (F3O7C) Rabbit Monoclonal Antibody recognizes endogenous levels of total Kir4.1 protein. This antibody detects a non-specific band at 70 kDa by western blot. Non-specific staining was observed in frozen formaldehyde fixed mouse lung by immunofluorescence.
Species Reactivity: Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro339 of mouse Kir4.1 protein.
Background
Inwardly rectifying potassium (Kir) channels containing Kir4.1 subunits (Kir4.1 channels) are expressed exclusively in glial cells in the central nervous system (CNS) (1). These channels provide a spatial buffering system for potassium ions, playing an important role in maintaining neuronal excitability (1-3). Astrocytic Kir4.1 channels have a pathophysiological role in epilepsy, Fragile X syndrome, chronic pain, and depression (2,4,5). Furthermore, Kir4.1 channels are expressed in oligodendrocytes. Oligodendric potassium clearance has been found critical for sustained axonal function and integrity (6).
Alternate Names
ATP-sensitive inward rectifier potassium channel 10; BIR; BIR10; BIRK; BIRK-1; inward rectifier K(+) channel Kir4.1; KCJ10; Kcnj10; Kcnj10 protein; Kir1; Kir1.2; Kir4; Kir4.1; potassium channel, inwardly rectifying subfamily J member 10; potassium inwardly-rectifying channel, subfamily J, member 10
