Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation
Specificity / Sensitivity
UbcH5C (D60E2) Rabbit Monoclonal Antibody detects endogenous levels of total UbcH5C protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp135 of human UbcH5C protein.
Background
Protein ubiquitination requires the concerted action of the E1, E2 and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through an ATP-dependent formation of a thiol ester with an E1 enzyme. The activated ubiquitin is then transferred to a thiol-group of an E2 ubiquitin-conjugation enzyme. The final step is the transfer of ubiquitin from E2 to an ε-amino group of a lysine residue on the target protein, a transfer mediated by ubiquitin-conjugating enzyme E3 (1). UbcH5C is a universally expressed E2 ubiquitin conjugating enzyme and member of the UbcH5 family that also includes UbcH5A and UbcH5B (2). Evidence suggests that UbcH5C plays an important role in regulating a number of signaling pathways by catalyzing the ubiquitination of key target proteins, including p53, PCNA, the IκB kinase protein NEMO, and the apoptosis inhibitor BRUCE (3-6). Gene expression profiles revealed increased expression of UbcH5C in meibomian cell carcinoma and oncocytic thyroid adenomas (7,8), while an RNAi screen reveals diffrential Ubc5HC in acute promyelocytic cells (9). These results suggest a potential role of UbcH5C in cell cycle control and tumorigenesis.
Alternate Names
(E3-independent) E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D3; E2(17)KB 3; E2(17)KB3; MGC43926; MGC5416; UB2D3; UBC4/5; UBC5C; UBCH5C; UBE2D3; Ubiquitin carrier protein D3; ubiquitin conjugating enzyme E2 D3; ubiquitin conjugating enzyme E2D 3; Ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2-17 kDa 3; Ubiquitin-conjugating enzyme E2(17)KB 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3
