Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Gremlin Antibody detects transfected levels of total gremlin protein.
Species Reactivity: Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Val170 of human gremlin. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Gremlin is a secreted antagonist of bone morphogenetic proteins that play important roles during development. Gene targeting generates gremlin deficient mice that die shortly after birth due to absent kidneys and defects in lung septation (1-3). Gremlin also plays a role in cardiomyogenesis and angiogenesis (4,5). TGF-β can induce gremlin expression under pathological conditions. Gremlin colocalizes with TGF-β in tubulointerstitial fibrosis associated with chronic allograft nephropathy and is expressed in diabetic nephropathy (6-8). Gremlin is widely expressed in cancer associated stromal cells and can enhance cancer cell proliferation (9).
Alternate Names
C15DUPq; Cell proliferation-inducing gene 2 protein; CKTSF1B1; colorectal adenoma and carcinoma 1; CRAC1; CRCS4; Cysteine knot superfamily 1, BMP antagonist 1; DAN domain family member 2; DAND2; Down-regulated in Mos-transformed cells protein; DRM; DUP15q; GREM1; GREMLIN; gremlin 1-like protein; gremlin 1, cysteine knot superfamily, homolog; gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis); gremlin 1, DAN family BMP antagonist; Gremlin-1; hereditary mixed polyposis syndrome; HMPS; HMPS1; IHG-2; Increased in high glucose protein 2; increased in high glucose-2; MGC126660; MPSH; PIG2; proliferation-inducing gene 2
