Description
Product Usage Information
This product is the carrier free version of product #73403. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 73403
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
Desmoplakin (F3J8Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total desmoplakin protein.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human desmoplakin protein.
Background
Desmosomes are a class of intracellular junctions that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein desmoplakin, which acts as a tether by binding the cytoplasmic component of the desmosome at its N-terminus (2) while its C-terminus is anchored to the intermediate-filament cytoskeleton (3). This association mitigates the impact of mechanical forces on the desmosome by distributing them throughout the cytoskeleton and tissue (4). Desmoplakin is essential for normal desmosomal adhesion (5); defects can result in pathologies that include cardiomyopathy (6), keratoderma (7), or the skin blistering disease Epidermolysis bullosa (8).
Alternate Names
250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; Desmoplakin; desmoplakin I; desmoplakin II; DESP; DP; DPI; DPII; DSP
