Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:200
Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800
Flow Cytometry (Live): 1:200 - 1:800
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live)
Specificity / Sensitivity
IL-1R2 (F5S6U) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-1R2 protein.
Species Reactivity: Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human IL-1R2 protein.
Background
IL-1R2 is a type I transmembrane glycoprotein that is expressed predominantly by cells of the myeloid lineage (1). Like IL-1R1, the extracellular domain of IL-1R2 is capable of binding to all three forms of IL-1 (2). Unlike IL-1R1, however, membrane-bound IL-1R2 has a short cytoplasmic tail and does not transduce signals upon binding IL-1 but rather functions as a decoy receptor to dampen IL-1 signaling (3,4). As a negative regulator of the IL-1 receptor signaling complex, research studies have also demonstrated that membrane-bound IL-1R2 undergoes regulated intramembrane proteolysis in response to pro-inflammatory molecules. This process converts the extracellular domain of IL-1R2 into a soluble decoy receptor capable of binding IL-1, limiting its capacity to elicit productive signals through membrane-bound IL-1R1 (5,6). Recent studies have demonstrated that some types of human cancers are enriched with regulatory T cells that express high levels of IL-1R2, an attribute that may contribute to their role in immunosuppression (7,8).
Alternate Names
antigen CDw121b; CD121 antigen-like family member B; CD121b; CDw121b; IL-1 type II receptor; IL-1R-2; IL-1R-beta; IL-1RB; IL-1RT-2; IL-1RT2; IL1R2; IL1R2c; IL1RB; interleukin 1 receptor type 2; interleukin 1 receptor type II variant 3; interleukin 1 receptor, type II; Interleukin-1 receptor beta; Interleukin-1 receptor type 2; Interleukin-1 receptor type 2, membrane form; Interleukin-1 receptor type 2, soluble form; Interleukin-1 receptor type II; MGC47725; mIL-1R2; mIL-1RII; sIL-1R2; sIL-1RII; type II interleukin-1 receptor, beta
