Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:10 - 1:50
Immunofluorescence (Frozen): 1:200 - 1:800
Immunofluorescence (Immunocytochemistry): 1:400
Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
dCK (F1F7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total dCK protein. Reactivity for immunofluorescence and flow cytometry is mouse only.
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys252 of mouse dCK protein.
Background
Deoxycytidine kinase (dCK) catalyzes the rate-limiting step in the nucleoside salvage pathway. dCK phosphorylates salvaged deoxycytidine (dC) to generate deoxycytidine monophosphate (dCMP), a precursor for both deoxycytidine triphosphate (dCTP) and deoxythymidine triphosphate (dTTP) pools (1). dCK also phosphorylates deoxyadenosine (dA) and deoxyguanosine (dG) to generate dA monophosphate and dG monophosphate, respectively. Gemcitabine is an anticancer nucleoside that is a dC analog. It is a pro-drug and must be phosphorylated by dCK to an active form. dCK is phosphorylated on Ser74 by the DNA damage signaling kinases ATM and ATR, and this phosphorylation increases dCK activity (2,3).
Alternate Names
Dck; Deoxyadenosine kinase; Deoxycytidine kinase; Deoxyguanosine kinase; OTTMUSP00000030586
