Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
ARK5 Antibody detects endogenous levels of total ARK5 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys403 of human ARK5 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
AMP-activated protein kinases (AMPKs) constitute a serine/threonine protein kinase family, which is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPKα1, AMPKα2, MELK, and SNARK are the catalytic subunits in the family (2). Recently, AMPK-related protein kinase ARK5 was identified, which shares 84% similarity with the sequence of the catalytic domain of SNARK (2). , Akt phosphorylates ARK5 at Ser600. This phosphorylation activates ARK5, resulting in increased phosphorylation of the SAMS peptide, an AMPK consensus substrate (2). experiments showed that Akt-activated ARK5 is critical for the survival of cells under glucose starvation (2). Furthermore, studies also linked ARK5 to tumor invasion (3,4). Overexpressed ARK5 leads to a higher tumor growth rate (3). For example, the overexpression of ARK5 in pancreatic tumor cell line PANC-1 significantly elevates its metastasis in the liver (4).
Alternate Names
AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; ARK5; KIAA0537; NUAK family kinase 1; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; NUAK1; Omphalocele kinase 1; OMPHK1
