Description
Product Usage Information
Western Blotting: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (Frozen): 1:400 - 1:800
Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
G3bp1 (E8N8F) Rabbit Monoclonal Antibody recognizes endogenous levels of total G3bp1 protein. This antibody does not cross-react with G3bp2 protein.
Species Reactivity: Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse G3bp1 protein.
Background
Ras GTPase-activating protein-binding protein 1, also known as GAP SH3 domain-binding protein 1 (G3BP1), was identified as a protein that interacts with the SH3 domain of Ras GTPase-activating protein (RasGap) (1). G3BP1 is involved in the regulation of multiple cellular processes, including mRNA decay and inhibition of translation initiation (2). Furthermore, G3BP1 is essential for the assembly of stress granules (SGs) and functions as an SG-nucleating protein (3). Research studies show that arginine demethylation of G3BP1 promotes SG assembly during oxidative stress (4).
Alternate Names
AI849976; ATP-dependent DNA helicase VIII; B430204O07; C87777; G3bp; G3BP-1; G3bp1; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; HDH-VIII; KIAA4115; mKIAA4115; OTTMUSP00000005871; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1
