Description
Product Usage Information
Western Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:50
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunohistochemistry (Paraffin)
Specificity / Sensitivity
Fumarase (D9C5) Rabbit Monoclonal Antibody recognizes endogenous levels of total fumarase protein.
Species Reactivity: Human, Mouse, Rat, Hamster, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly354 of human fumarase protein.
Background
Fumarate hydratase (FH), also known as fumarase, is canonically regarded as a mitochondrial enzyme of the tricarboxylic acid cycle that catalyzes fumarate to malate (1,2). Studies have demonstrated that deficiencies in FH can lead to hereditary leiomyomatosis and renal cell cancer (1,3) or, in homozygous germline mutations, severe neurological and muscular disorders (1,2). A cytosolic fumarase isoenzyme is also involved in DNA damage repair (3).
Alternate Names
epididymis secretory sperm binding protein; FH; FMRD; Fumarase; fumarate hydratase; Fumarate hydratase, mitochondrial; FUMH; HLRCC; HsFH; LRCC; MCL; MCUL1
