Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
HPRT1 (F1O9R) Rabbit Monoclonal Antibody recognizes endogenous levels of total HPRT1 protein.
Species Reactivity: Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HPRT1 protein.
Background
Hypoxanthine phosphoribosyltransferase 1 (HPRT1) is a key enzyme in the purine salvage pathway. Studies show that, in the presence of the PI3K/mTOR dual inhibitor gedatolisib, reduced HPRT1 expression leads to decreased survival rate of small-cell lung carcinoma (SCLC) cells, suggesting that HPRT1 plays a role in SCLC cell sensitivity to gedatolisib (1). Furthermore, electron transport chain (ETC) inhibition suppresses purine synthesis and enhances the purine salvage pathway. HPRT1 was shown to be important for non-small cell lung cancer (NSCLC) growth when ETC is inhibited (2). In addition, HPRT1 is involved in the metabolism of temozolomide (TMZ) to activate AMPK and promote chemoresistance of glioblastomas (GBMs) (3).
Alternate Names
HGPRT; HGPRTase; HPRT; HPRT1; hypoxanthine phosphoribosyltransferase 1; Hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89
