Description
Product Usage Information
Western Blotting: 1:1000
Immunofluorescence (Immunocytochemistry): 1:100
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
PRPF3 (F8X1N) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRPF3 protein.
Species Reactivity: Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu672 of human PRPF3 protein.
Background
Pre-mRNA processing factor 3 (PRPF3) is a U4/U6 snRNP-associated protein that is a critical regulator of the splicing process (1-3). PRPF3, PRPF4, and PPIH form a stable complex essential for the proper formation and function of the U4/U6.U5 tri-snRNP (3). PRPF3 interacts with SPF30, which forms a bridge between tri-snRNP and U2AF35, a protein that recognizes 3' splice sites (4). mutations cause autosomal dominant retinitis pigmentosa (5,6). Furthermore, has been identified as a potential oncogene in hepatocellular carcinoma (7).
Alternate Names
HPRP3; HPRP3P; pre-mRNA processing factor 3; Pre-mRNA-splicing factor 3; PRP3; PRP3 pre-mRNA processing factor 3 homolog; PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae); Prp3p; PRPF3; RP18; SNRNP90; U4/U6 small nuclear ribonucleoprotein Prp3; U4/U6 snRNP 90 kDa protein; U4/U6-associated RNA splicing factor
