Description
Product Usage Information
Western Blotting: 1:1000
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting
Specificity / Sensitivity
Bik Antibody detects endogenous levels of Bik protein. This antibody does not cross-react with other Bcl-2 family members.
Species Reactivity: Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with synthetic peptide corresponding to residues surrounding Asp40 of human Bik protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Bik/Nbk (Bcl-2-interacting killer/natural born killer) is a potent pro-apoptotic protein belonging to a group of Bcl-2 family members that includes Bad, Bid, Bim, Hrk, and Noxa, containing a BH3 domain but lacking other conserved domains, BH1 or BH2 (1,2). Functionally, Bik is able to bind to and antagonize anti-apoptotic Bcl-2 family members including Bcl-2, Bcl-xL, and viral homologs E1B-19K and EBV-BHFR1. The BH3 domain of Bik is essential for its apoptotic activity and interaction with survival proteins (3). Phosphorylation of Bik is correlated with an increase in its pro-apoptotic activity (4).
Alternate Names
Apoptosis inducer NBK; apoptosis-inducing NBK; Bcl-2-interacting killer; BCL2 interacting killer; BCL2-interacting killer (apoptosis-inducing); BIK; BIP1; BP4; natural born killer; NBK
