Description
Product Usage Information
Western Blotting: 1:1000
Simple Western™: 1:50 - 1:250
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:1500 - 1:6000
Immunofluorescence (Frozen): 1:50 - 1:200
Immunofluorescence (Immunocytochemistry): 1:1600
Flow Cytometry (Fixed/Permeabilized): 1:3200 - 1:6400
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.
Protocol
Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized)
Specificity / Sensitivity
TCF4/ITF2 (E3X2Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total TCF4/ITF2 protein. This antibody is not recommended for immunohistochemical analysis of human tissues. This antibody is predicted to detect all 16 isoforms of human TCF4/ITF2 protein produced by alternative splicing described on Uniprot (P15884-1 through P15884-16).
Species Reactivity: Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro653 of human TCF4/ITF2 protein.
Background
Transcription factor 4 (TCF4), also known as immunoglobulin transcription factor 2 (ITF2), is a member of the basic helix-loop-helix family and interacts with Ephrussi-box (E-box) binding sites (1,2). TCF4 is broadly expressed, and due to alternative splicing, transcripts can give rise to over a dozen different isoforms (3). It plays a key role in neuronal development and has been implicated in a variety of developmental disorders, including Pitt-Hopkins syndrome, schizophrenia, and autism (4-9). In addition to its functions in the brain, TCF4 is essential for the development of plasmacytoid dendritic cells (10-12). Finally, in the context of cancer, TCF4 expression may be associated with divergent patient outcomes; higher TCF4 expression is associated with increased survival in non-small cell lung cancer, yet the protein has also recently been reported to confer immunotherapy resistance in melanoma (13,14).
Alternate Names
BHLHB19; CDG2T; Class B basic helix-loop-helix protein 19; E2-2; FECD3; Immunoglobulin transcription factor 2; ITF-2; ITF2; MGC149723; MGC149724; PTHS; SEF-2; SEF2; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; SL3-3 enhancer factor 2; TCF-4; TCF4; Transcription factor 4
